Drug expiration dating calculations
Calculating appropriate dilutions and doses for mouse injectable analgesics and anesthetics can be an intricate and time-consuming process.
Determining the correct amount to administer to a mouse is dependent on several variables including animal weight, acceptable injection volume, and simultaneous administration of multiple drug agents.
Users combine output volumes in Step 5 (e) and inject a volume of 0.3m L via routes suggested in Steps 6 (f).
The Mouse Dosage Calculator The Mouse Dosage Calculator is a computer-based spreadsheet application designed using the Microsoft Excel 2010 software platform.
Animal size is a crucial variable because relatively small injection volumes and the high variability of mouse weights, particularly in older animals, allows for a greater likelihood of inadvertent under- or overdosing.
In order to determine appropriate administration of drug agents, health care professionals employ the formula below to first determine the amount of the original drug stock solution needed.
Desired Dose (mg/kg) Animal Weight (kg)/Final Volume (m L)=Working Concentration (mg/m L) When performing simultaneous administration of multiple drugs, the dilutant volume and working concentrations of each drug are modified to take into account the relative changes in drug agent volumes.
The agents selected in this calculator should therefore be labeled with an expiration date of no more than 28 days, and discarded after this timeframe.The calculator has been field tested by veterinary technicians within the University of California, San Francisco (UCSF) Laboratory Animal Resource Center on a range of mouse cases.The Challenges of Compounding Commercially produced preparations of drugs in concentrations ideal for mice are not readily available.For example, it would be unreasonable to administer a 5m L injection to a mouse as there is simply not enough room inside the animal to contain this volume.Post-dilution, the working concentration of this solution is calculated using the formula shown below.